Laboratories:


1. Preparation and characterization of liposomes.

Students will receive hands-on training in handling phspholipids: storage, stock solution preparation and storage, liposome preparation and storage, liposome characterization by dynamic light scattering and z-potential measurements..

Tutors: Ling Zhu, Swati Gupta.

2. A basic quartz crystal microbalance experiment:

Supported lipid bilayer formation versus vesicle adsorption. Surface preparation and cleaning, experimental setup and data evaluation.

Tutors: Marta Gallego, Leire Diaz, Patricia Wolny.

3. Combined quartz crystal microbalance/ellipsometry measurements of bilayer formation and liposome adsorption on streptavidin monolayers.

Acoustic versus optical methods.

Tutors: Ralf Richter, Nico Eisele, Natalia Baranova.

4. Fluorescence methods for characterizing supported lipid systems:

Fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, fluorescence lifetime imaging.

Tutors: Aleš Benda, Radek Machan, Marie Kolarova.

5. Measuring forces with atomic force microscopy.

Tutors: Ralf Richter, Seetharamaiah Attili, Ilya Reviakine, Sandra Camarero, Pablo Mateos-Gil, Marisela Velez.

6. Scanning and transmission electron microscopy:

Imaging particles and structured surfaces at the nanoscale.

Tutors: Marco Möller.

7. Demonstration of an 8-cell ellipsometer:

Estimating high-affinity binding constants in solution or on membranes.

Tutors: Wim Hermens, Han Speijer.

8. Basics of Flow Cytometry:

Tutors: Swati Gupta.

9. Basics of Surface Analysis :

Tutors: Ilya Reviakine.